Gamete Intra – Fallopian Transfer (GIFT)
- It was first described by Aschetal in 1984.It involves aspiration of oocytes following ovulation induction either laproscopically or under ultrasound guidance transvaginally.
- Laparoscopic route is preferred as it anyway requires sperm & oocyte transfer in to the fallopian tube.
- 2hours before aspiration, semen is prepared, washed from the seminal plasma * remained in culture medium at 37 c.
- The oocytes (2 per tube) are mixed with 50,000 sperms, &transferred to each ampullary portion of the fallopian tube 4 cm from the fimbrial end.
- The volume transferred is to 10 to 20µ (micron).
- Lately, transfer of oocytes & sperm are attempted by transuterine catheterization of the tube (falloscopically).
Indications are :-
- Un explained infertility
- Failed intra uterine insemination
- Immunological factor in male
- Immunological factors in cervix
- Donor semen required (rare)
→Both the fallopian tubes must be patent. The results are better with GIFT IBF, I.e. 45%S success versus 15 to 20%.Abortion rate of 10 to 15%, ectopic pregnancy (7%) & multiple pregnancy (20 to 50%) have been reported.